量研学術機関リポジトリ「QST-Repository」は、国立研究開発法人 量子科学技術研究開発機構に所属する職員等が生み出した学術成果（学会誌発表論文、学会発表、研究開発報告書、特許等）を集積しインターネット上で広く公開するサービスです。 Welcome to QST-Repository where we accumulates and discloses the academic research results(Journal Publications, Conference presentation, Research and Development Report, Patent, etc.) of the members of National Institutes for Quantum and Radiological Science and Technology.
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Purpose:The Purpose of this study was to assess the gene expression changes in oral squamous cell carcinoma (OSCC) cells after carbon ion irradiation.
Methods and Materials: Three OSCC cell lines (HSC2, Ca9-22, and HSC3) were irradiated with accelerated carbon ion beams or X-rays using three different doses.
The cellular sensitivities were determined by clonogenic survival assay.To idenify genes the expression of which is influenced by carbon ion irradiation in a dose-dependent manner. we performed Affymetrix GeneChip analysis with HG-U133 plus 2.0 arrays containing 54,675 probe sets. The idenified genes were analyzed using the Ingenuity Pathway Analusis Tool to investigate the functional network and gene outology. changes in mRNA expression in the genes were assessed by real-time reverse transcriResults: We identified 98 genes with expression levels that were altered sibnificantly at least twofold in each of the three cabon-irradiated OSCC cell lines at all dose points compared with nonirradiated control cells. Among these, SPHK1,the expression of which was significantly upregulated by carbon ion irradiatin, was modulated little by X-rays. The function of SPHK1 related to cellular growth and proliferation had the highest p value (p=9.25e-7 to 2.19e-2).Real-time reverse transcriptase-polymerase chain reaction analysis showed significantly elevated SPHK1 expression leaves after carbon ion irradiation (p < 0.05), consistent with microarray date. Clonogenic survival assay indicated that carbon ion irradiation could induce cell death in Ca0-22 cells more effectively than X-rays.
Conclusions: Our findings suggest that SPHK1 helps to elucidate the molecuar mechanisims and processes underlying the biologic response to carbon ion beams in OSCC.ptase-polymerase chain reaction.
雑誌名
International Journal of Radiation Oncology Biology Physics
ENHANCEMENT OF SPHK1 IN VITRO BY CARBON ION IRRADIATION IN ORAL SQUAMOUS CELL CARCINOMA
