WEKO3
アイテム
{"_buckets": {"deposit": "caf4c510-afa4-4d85-bc48-8b301d143d3b"}, "_deposit": {"created_by": 1, "id": "43766", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "43766"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00043766", "sets": ["1"]}, "author_link": ["435171", "435173", "435170", "435172", "435168", "435169"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2004", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "318", "bibliographicPageStart": "311", "bibliographicVolumeNumber": "44", "bibliographic_titles": [{"bibliographic_title": "Journal of Radiation Research"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The growth arrest and DNA damage-inducible protein 45alpha(GADD45a)gene is responsive to a variety of DNA-damaging agents. It is known that induction of the GADD45a gene is regulated in a p53-dependent manner after ionizing irradiation. Our previous study showed that X-ray irradiation increased the transcription rate of the GADD45a gene much earlier than the maximum accumulation of stabilized p53 protein in human myeloblastic leukemia ML-1 cells. We hypothesized that some transcription factor(s) may cooperate with p53 in regulating the GADD45a gene early after the irradiation of ML-1 cells. This idea is supported by recent studies showing that the p53-dependent activation of several genes in human and mouse cells requires some additional transcription factors, such as Sp1, GKLF, Ets1, and IRF-1. To examine the possible involvement of cooperating factors in transcriptional regulation of the GADD45a gene by ionizing radiation, we complehensively searched for the X-ray-inducible binding locus of the nuclear factor throughout the upstream region (-2244 bp/+89 bp) and the third intron (+1389 bp/+2488 bp) of the GADD45a gene by EMSA using 136 probes. The X-ray-responsive binding of nuclear factors was detected at eight loci. Oct, NF-kappaB, HNF, NF-AT, and KLF family transcription factors were identified by a competition assay. It is possible that some of these factors cooperate with p53 to mediate transcriptional regulation of the GADD45a gene after ionizing irradiation.", "subitem_description_type": "Abstract"}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0449-3060", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Daino, Kazuhiro"}], "nameIdentifiers": [{"nameIdentifier": "435168", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ichimura, Sachiko"}], "nameIdentifiers": [{"nameIdentifier": "435169", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nenoi, Mitsuru"}], "nameIdentifiers": [{"nameIdentifier": "435170", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "臺野 和広", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "435171", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "沼田 幸子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "435172", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "根井 充", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "435173", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Comprehensive search for X-ray-responsive elements and the binding factors in the regulatory region of the GADD45a gene", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Comprehensive search for X-ray-responsive elements and the binding factors in the regulatory region of the GADD45a gene"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/43766", "pubdate": {"attribute_name": "公開日", "attribute_value": "2005-08-11"}, "publish_date": "2005-08-11", "publish_status": "0", "recid": "43766", "relation": {}, "relation_version_is_last": true, "title": ["Comprehensive search for X-ray-responsive elements and the binding factors in the regulatory region of the GADD45a gene"], "weko_shared_id": -1}
Comprehensive search for X-ray-responsive elements and the binding factors in the regulatory region of the GADD45a gene
https://repo.qst.go.jp/records/43766
https://repo.qst.go.jp/records/43766c7658a28-2a87-49d9-8c12-7a375994be3e
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2005-08-11 | |||||
タイトル | ||||||
タイトル | Comprehensive search for X-ray-responsive elements and the binding factors in the regulatory region of the GADD45a gene | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Daino, Kazuhiro
× Daino, Kazuhiro× Ichimura, Sachiko× Nenoi, Mitsuru× 臺野 和広× 沼田 幸子× 根井 充 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The growth arrest and DNA damage-inducible protein 45alpha(GADD45a)gene is responsive to a variety of DNA-damaging agents. It is known that induction of the GADD45a gene is regulated in a p53-dependent manner after ionizing irradiation. Our previous study showed that X-ray irradiation increased the transcription rate of the GADD45a gene much earlier than the maximum accumulation of stabilized p53 protein in human myeloblastic leukemia ML-1 cells. We hypothesized that some transcription factor(s) may cooperate with p53 in regulating the GADD45a gene early after the irradiation of ML-1 cells. This idea is supported by recent studies showing that the p53-dependent activation of several genes in human and mouse cells requires some additional transcription factors, such as Sp1, GKLF, Ets1, and IRF-1. To examine the possible involvement of cooperating factors in transcriptional regulation of the GADD45a gene by ionizing radiation, we complehensively searched for the X-ray-inducible binding locus of the nuclear factor throughout the upstream region (-2244 bp/+89 bp) and the third intron (+1389 bp/+2488 bp) of the GADD45a gene by EMSA using 136 probes. The X-ray-responsive binding of nuclear factors was detected at eight loci. Oct, NF-kappaB, HNF, NF-AT, and KLF family transcription factors were identified by a competition assay. It is possible that some of these factors cooperate with p53 to mediate transcriptional regulation of the GADD45a gene after ionizing irradiation. | |||||
書誌情報 |
Journal of Radiation Research 巻 44, p. 311-318, 発行日 2004 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0449-3060 |