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Kinetics of in vitro fertilization and development of an inbred mouse strain: study using RFM/Ms compared with C57BL/6J.
https://repo.qst.go.jp/records/42883
https://repo.qst.go.jp/records/42883d8bf82cf-4a68-4d9a-9ab8-83b36f218487
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2002-05-31 | |||||
タイトル | ||||||
タイトル | Kinetics of in vitro fertilization and development of an inbred mouse strain: study using RFM/Ms compared with C57BL/6J. | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Kito, Seiji
× Kito, Seiji× Tateno, Shintarou× Oota, Yuki× Noguchi, Yoshiko× 鬼頭 靖司× 舘野 真太郎× 太田 有紀 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In vitro fertilizability and subsequent developmental competence of inbred RFM/Ms mice, which have high incidence of myeloid leukemia by radiation, was studied. When response of RFM/Ms females to various amount of eCG (1.25-10.0 i.u.) was examined, superovulation (>20 ovulated ova) occurred in animals injected with ³5.0 i.u. eCG. The kinetics of in vitro fertilization and embryo development were examined and compared among RFM/Ms, C57BL/6J zygotes and their hybrid zygotes. The fertilization kinetics was significantly slower in RFM/Ms zygotes than in C57BL/6J zygotes. Crossbreeding experiments between C57BL/6J and RFM/Ms showed that the speed and incidence of fertilization were affected by paternal and maternal factors, respectively. The in vitro development to the blastocyst stage of RFM/Ms zygotes was significantly slower than that of C57BL/6J zygotes and their hybrid zygotes at 96h post-insemination (PI), but not at 120h PI. The nuclear number of RFM/Ms embryos was significantly lower than that of C57BL/6J and their hybrid embryos throughout the culture period (72, 96 and 120h PI). Conversely, hatching at 120h PI in C57BL/6J embryos was much slower than that of the other groups of embryos. This study showed that gametes and zygotes of RFM/Ms strain can be fertilized and developed well in vitro and that maternal and paternal factors and their interaction have complicated effects on fertilization and developmental kinetics in vitro. | |||||
書誌情報 |
Journal of Mammalian Ova Research 巻 19, p. 32-38, 発行日 2002-04 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1341-7738 |